期刊
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 75, 期 5, 页码 557-568出版社
WILEY
DOI: 10.1111/aji.12506
关键词
advanced glycation end products; high-mobility group box-1; inflammation; placenta
资金
- Japan Society for the Promotion of Science (JSPS)
- Tokyo University of Agriculture
- Grants-in-Aid for Scientific Research [15K07783] Funding Source: KAKEN
ProblemAdvanced glycation end products (AGEs) and high-mobility group box-1 (HMGB1) are considered contributing to placental inflammation. We examined the effect of AGEs and HMGB1 on cytokines from Sw.71 human trophoblast cell lines and the interactions between Sw.71 cells and THP-1-monocytes. Methods of studySw.71 cells were cultured with/without AGEs or HMGB1. We examined the role of AGEs or HMGB1 on THP1 migration and effect of AGEs on IL-6 from Sw.71 cells using co-cultures or conditioned medium from THP-1 cells. ResultsAGEs and HMGB1 increased interleukin (IL)-6, IL-8, and chemokine C-C motif ligand 2 (CCL2) secretion from Sw.71 cells. The secretion of IL-6 was dependent on reactive oxygen species (ROS) and NF-B. AGEs stimulated IL-6 secretion through receptor RAGE and TLR4, whereas HMGB1 stimulated it through TLR4. AGEs, but not HMGB1, increased monocyte migration via IL-8 and CCL2 from Sw.71 cells. THP-1 monocytes induced IL-6 secretion from Sw.71 cells, and AGEs further stimulated it. ConclusionsAGEs and HMGB1 may promote sterile placental inflammation cooperating with monocytes/macrophages.
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