期刊
FOOD CHEMISTRY
卷 277, 期 -, 页码 135-144出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.10.071
关键词
Keywords: Flour proteome; Gluten proteins; Grain hardness; iTRAQ (isobaric tag for relative and absolute quantitation); Nitrogen topdressing timing; Wheat
资金
- China Scholarship Council (CSC)
- National Key Research and Development Program of China [2016YFD0300408, 2016YFD0300107]
- National Natural Science Foundation of China [31325020, 31401326, 31471445]
- China Agriculture Research System [CARS-03]
- Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP)
- National Non-profit Program by the Ministry of Agriculture [201403039]
- 111 Project [B16026]
Nitrogen fertilization regimes significantly affect both grain quality and yield. Wheat plants were subjected to different application timing of topdressed nitrogen at the emergence of the top fifth (TL5), top third (TL3) and top first leaf (TL1), respectively. The iTRAQ (isobaric tag for relative and absolute quantitation) technology was adopted to obtain the complete proteome of wheat flour and to identify the differentially expressed proteins (DEPs) as regulated by nitrogen topdressing timing. Collectively, 591 proteins into 17 functional categories in flour of mature grains were identified. In comparison to TL3, 50 and 63 DEPs were identified in TL5 and TL1, respectively. Nine of the DEPs commonly dependent on nitrogen topdressing timing are the gamma-gliadins or high-molecular-weight glutenin subunits. Additionally, delaying nitrogen topdressing modified the grain hardness and allergic protein content. The results suggested that altering nitrogen topdressing timing is a potential strategy for pursuing targeted processing quality of wheat flour.
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