期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 124, 期 -, 页码 324-335出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2018.12.022
关键词
Ceramide; Diglyceride; Apoptosis; Endoplasmic reticulum stress; Diabetes; Trans fatty acids
资金
- Hungarian National Research, Development and Innovation Office (NKFIH) [K 125201]
- Higher Education Excellence Program of the Ministry of Human Capacities in Biotechnology research area of Budapest University of Technology and Economics (BME FIKP-BIG)
- Hungarian Academy of Sciences
High fatty acid (FA) levels are deleterious to pancreatic beta-cells, largely due to the accumulation of biosynthetic lipid intermediates, such as ceramides and diglycerides, which induce ER stress and apoptosis. Toxicity of palmitate (16:0) and oleate (18:1 cis-Delta(9)) has been widely investigated, while very little data is available on the cell damages caused by elaidate (18:1 trans-Delta(9)) and vaccenate (18:1 trans-Delta(11)), although the potential health effects of these dietary trans fatty acids (TFAs) received great publicity. We compared the effects of these four FAs on cell viability, apoptosis, ER stress, JNK phosphorylation and autophagy as well as on ceramide and diglyceride contents in RINm5F insulinoma cells. Similarly to oleate and unlike palmitate, TFAs reduced cell viability only at higher concentration, and they had mild effects on ER stress, apoptosis and autophagy. Palmitate increased ceramide and diglyceride levels far more than any of the unsaturated fatty acids; however, Incorporation of TFAs in ceramides and diglycerides was strikingly more pronounced than that of oleate. This indicates a correlation between the accumulation of lipid intermediates and the severity of cell damage. Our findings reveal important metabolic characteristics of TFAs that might underlie a long term toxicity and hence deserve further investigation.
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