期刊
FOOD AND BIOPROCESS TECHNOLOGY
卷 12, 期 6, 页码 910-918出版社
SPRINGER
DOI: 10.1007/s11947-019-02270-6
关键词
Abalone viscera; Subcritical water extraction; Enzymatic hydrolysis; Proximate composition; Antioxidant activities
资金
- National Natural Science Fund [31571835]
- Special Scientific Research Fund of Marine Public Welfare [201405016]
- Xiamen Science and Technology Project [3502Z20173032]
Antioxidant hydrolysates were prepared from abalone viscera using subcritical water (AVS)-assisted by enzymatic hydrolysis with papain (AVSE-P), bromelain (AVSE-B), neutral protease (AVSE-N), and flavourzyme (AVSE-F). The protein and carbohydrate contents reached 38.33% and 24.36%, respectively. When AVS was digested by any of the proteases, the protein content increased, but carbohydrate content decreased. The main amino acids of AVSEs included alanine, glycine, and aspartic acid. The IC50 values of ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH)(center dot), 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)(center dot+), N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD)(center dot+), and hydroxyl radical ((OH)-O-center dot) scavenging abilities of AVS were 2.93, 1.48, 1.61, 3.72, and 5.51 mg/mL, respectively, which decreased after enzymatic hydrolysis by any of the proteases. The DMPD center dot+ and (OH)-O-center dot scavenging abilities of AVSE-P and AVSE-B were higher than those of others, whereas the opposite was observed in lipid peroxidation inhibition efficiency, DPPH center dot, and ABTS(center dot+) scavenging abilities. Hence, antioxidant activities of AVS could be enhanced by enzymatic hydrolysis, but the influence depends on the type of protease. At the same time, results also suggest that the proposed approach can be used for treating abalone viscera, and the obtained antioxidant hydrolysates could be used in nutraceutical and pharmaceutical industries.
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