期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 126, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2019.03.006
关键词
Bisphenol A; Laccase; Mesoporous silica; Crosslinking; Fluidized bed reactor
资金
- National Natural Science Foundation of China [51809111, 41572213]
- Natural Science Foundation from the Department of Science & Technology of Jilin Province of China [20150101094JC]
- Science and Technology Department of Siping City [2016053]
- Science development project in Jilin Province [2017031201ZX]
We developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Also, the SER/Lac presented better reusability compared to ADS/Lac, and it could be reused for three times without decrease of biotransformation efficiency, and half of it was remained after ten times use. Due to great stability and robustness property, SER/Lac was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.
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