期刊
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
卷 38, 期 5, 页码 1052-1061出版社
WILEY
DOI: 10.1002/etc.4375
关键词
acetylcholinesterase inhibitors; bioconcentration; ecotoxicology; herbicide; stress response
资金
- Intramural EPA [EPA999999] Funding Source: Medline
Tank mixtures are popular within the agricultural community because they are time- and cost-effective, but field applications leave nontarget organisms at risk of exposure. We explored the effects of a common herbicide (atrazine and alachlor) and fertilizer (urea) tank mixture on juvenile frog corticosterone stress levels, acetylcholinesterase (AChE) activity, and pesticide bioaccumulation. Single agrochemical or tank mixtures were applied to terrestrial microcosms, and then individual Southern leopard frog (Lithobates sphenocephala) juveniles were added to microcosms for an 8-h exposure. Afterward, frogs were transferred to aquatic microcosms for 1 h to monitor corticosterone prior to euthanasia, brain tissues were excised to evaluate AChE, and tissue homogenates were analyzed for pesticide bioconcentation with gas chromatography-mass spectrometry. Atrazine significantly increased corticosterone in frogs, particularly when combined with alachlor and urea. Atrazine increased AChE and urea decreased AChE, although no interactive effects of chemical combinations were discernible. Relative to their individual treatments, the complete tank mixture with all 3 agrochemicals resulted in 64% greater bioconcentration of atrazine and 54% greater bioconcentration of alachlor in frog tissues. Our results suggest that agrochemical mixtures as well as their active ingredients can lead to altered stress levels and impaired physiological responses in amphibians. An improved understanding of the effects of co-exposure to environmental contaminants in amphibians is important in assessing the ecological risks these compounds pose. Environ Toxicol Chem 2019;9999:1-10. (c) 2019 SETAC
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