期刊
EMBO JOURNAL
卷 38, 期 11, 页码 -出版社
WILEY
DOI: 10.15252/embj.201899630
关键词
actin; cell adhesion; centrosome; microtubule
资金
- Uehara Memorial Foundation (Japan)
- Fondation pour la Recherche Medicale [FDT20150532056]
- Association Nationale pour la Recherche [ANR-PoLyBex-12-BSV3-0014-001, ANR-Mitotube-12-BSV5-0004-01]
- European Research Council [ERC-Strapacemi-GA 243103, ERC-SpiCy 310472]
The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here, we show that increasing densities of actin filaments at the centrosome of lymphocytes are correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in disassembly of centrosomal actin and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. These two assays demonstrated that actin filaments constitute a physical barrier blocking elongation of nascent microtubules. Finally, we showed that cell adhesion and cell spreading lead to lower densities of centrosomal actin, thus resulting in higher microtubule growth. We therefore propose a novel mechanism, by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture.
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