期刊
EMBO JOURNAL
卷 38, 期 9, 页码 -出版社
WILEY
DOI: 10.15252/embj.201899839
关键词
calcium waves; hair cells; pre-hearing development; purinergic receptors; spontaneous activity
资金
- Wellcome Trust [102892/Z/13/Z]
- National Institute on Deafness and Other Communication Disorders [K18 DC013304]
- 2015-2016 Fulbright Scholar Award
- DFG [CRC 894, CRC 1027]
- MRC [MR/K005561/1]
- MRC [MR/K005561/1] Funding Source: UKRI
- Wellcome Trust [102892/Z/13/Z] Funding Source: Wellcome Trust
Outer hair cells (OHCs) are highly specialized sensory cells conferring the fine-tuning and high sensitivity of the mammalian cochlea to acoustic stimuli. Here, by genetically manipulating spontaneous Ca2+ signalling in mice invivo, through a period of early postnatal development, we find that the refinement of OHC afferent innervation is regulated by complementary spontaneous Ca2+ signals originating in OHCs and non-sensory cells. OHCs fire spontaneous Ca2+ action potentials during a narrow period of neonatal development. Simultaneously, waves of Ca2+ activity in the non-sensory cells of the greater epithelial ridge cause, via ATP-induced activation of P2X(3) receptors, the increase and synchronization of the Ca2+ activity in nearby OHCs. This synchronization is required for the refinement of their immature afferent innervation. In the absence of connexin channels, Ca2+ waves are impaired, leading to a reduction in the number of ribbon synapses and afferent fibres on OHCs. We propose that the correct maturation of the afferent connectivity of OHCs requires experience-independent Ca2+ signals from sensory and non-sensory cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据