期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 99, 期 -, 页码 5-10出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2018.12.008
关键词
Cerium oxide nanoparticle; Oxidase-mimicking activity; Biosensor; DNA; Electrochemical detection
资金
- BioNano Health-Guard Research Center - Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project [H-GUARD_2013M3A6B2078964]
- Mid-career Researcher Support Program of the National Research Foundation (NRF) - Ministry of Science and ICT (MSIT) of Korea [NRF-2018R1A2A1A05022355]
- Nano.Material Technology Development Program of the National Research Foundation (NRF) - Ministry of Science and ICT (MSIT) of Korea [2017M3A7B4041975]
We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3', 5,5'-tetramethylbenzidine (TMB) substrate, limits the formation of CeO2-TMB complex that is required for the oxidase-like reaction. As a result, the oxidase-mimicking activity of CeO2 is not effectively exerted, leading to the significantly diminished electrochemical current signal. With this novel strategy, we successfully determined DNA amplicon derived from Escherichia coli (E. coli) with the total assay time less than 6 min even without the post-purification of DNA amplicon.
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