期刊
DYES AND PIGMENTS
卷 162, 期 -, 页码 189-195出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2018.10.007
关键词
Sensor; Cell-imaging; BODIPY; Hypochlorite; Knoevenagel
资金
- Thailand Research Fund [RSA6080018, RTA6180007]
- National Nanotechnology Center, NSTDA, Ministry of Science and Technology, Thailand, through its program of Research Network NANOTEC (RNN)
- Chulalongkorn University
- Grant for International Research Integration: Chula Research Scholar
Fluorescent probes are great tools for visualization and imaging of reactive oxygen species (ROS) in living cells. Although several probes perform well with green emission, however, to maximize tissue penetration and minimize interference from auto-fluorescence, a probe with longer wavelength is desirable. In this study, boron-dipyrromethene (BODIPY)-based fluorescent probe SB2 with high cell penetration and ROS selectivity, has been developed for detection of intracellular ROS. SB2 has two styryl groups anchored to the BODIPY pyrrole rings to extend the emission wavelength into the red region (657 nm) and also to act as a new receptor for ROS. In aqueous media, the probe demonstrated colorimetric change from blue to purple and orange fluorescence turn on at 590 nm, selectively to hypochlorite, with the detection limit of 0.26 mu M. The several oxidative intermediates were observed by mass spectrometry. Fluorescence detection with SB2 of endogenous ROS, in RAW264.7 macrophages, induced by lipopolysaccharide (LPS) or addition of exogenous NaOCl was observed in the orange field (570-620 nm).
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