期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 92, 期 -, 页码 193-198出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.11.015
关键词
PEPCK-M; Silkworm; BmNPV; Antiviral; ATGs
资金
- National Natural Science Foundation of China [31501875, 31802014]
- Natural Science Foundation of Chongqing, China [cstc2018jcyjAX0211, cstc2016jcyjA0524, cstc2018jcyjAX0487]
- Chongqing Postdoctoral Science Foundation [XmT2018006]
Phosphoenolpyruvate carboxykinase (PEPCK) has cytoplasmic isoform (PEPCK-C) and a mitochondrial isoform (PEPCK-M). PEPCK-C plays an important role in gluconeogenesis, but the function of PEPCK-M is largely unknown. In this study, we cloned two isoforms of PEPCK (BmPEPCK-1 and BmPEPCK-2; both of PEPCK-M) from the lepidopteran model Bombyx mori. BmPEPCK-1 and BmPEPCK-2 were adjacently located in the silkworm genome, and both contained 13 exons. The main difference in the sequences was the 13th exon and 3'UTR. The expression of BmPEPCK-1 was higher than that of BmPEPCK-2, the overexpression of which did not affect BmNPV proliferation. The expression levels of BmPEPCK-2 and ATG6/7/8/13 decreased after BmNPV infection. Overexpression of BmPEPCK-2 increased the expression of ATG6/7/8 and significantly decreased viral fluorescence and content, suggesting that BmPEPCK-2 suppressed the multiplication of BmNPV by increasing ATGs expression. These results revealed that PEPCK-M has an important function in antiviral immunity.
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