期刊
CYTOMETRY PART A
卷 95A, 期 7, 页码 722-725出版社
WILEY
DOI: 10.1002/cyto.a.23753
关键词
cytometry; human PBMC; T cells; MAIT cells; gamma delta T cells; NK cells; memory; intracellular cytokines
资金
- Bill and Melinda Gates Foundation [OPP1066048, OPP1088952, OPP1099507]
- National Institute of Allergy and Infectious Diseases [P30 AI027757, UM1 AI068618]
- University of Washington/Fred Hutch Center for AIDS Research [P30 AI027757]
- Bill and Melinda Gates Foundation [OPP1088952, OPP1099507, OPP1066048] Funding Source: Bill and Melinda Gates Foundation
A 26-color staining panel was developed to profile human antigen-specific T cells in an intracellular cytokine staining (ICS) assay using peptide pools to various antigens of interest. In addition to multiple functional markers, the panel includes differentiation/activation markers and markers to assess gamma delta, mucosal-associated invariant T, and NK T cells as well as conventional NK cells. Panel optimization was performed using previously cryopreserved PBMC from healthy adults, and then, expression of key functional markers in the panel was cross-validated against a validated ICS assay used in the HIV Vaccine Trials Network (HVTN). The panel is currently being used to evaluate the responses to tuberculosis and malaria vaccine candidates in volunteers from different geographic areas. (C) 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
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