4.6 Article

Glucose uptake and lipid metabolism are impaired in epicardial adipose tissue from heart failure patients with or without diabetes

出版社

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00384.2015

关键词

epicardial adipose tissue; heart failure; diabetes; glucose uptake; lipolysis

资金

  1. SPD/GIFT award
  2. European Foundation for the Study of Diabetes [SFRH/BPD/26837/2006, PTDC/SAU-OSM/104124/2008, EXCL/DTP-PIC/0069/2012]
  3. Strategic Project - FEDER through Operational Programme Competitiveness Factors-COMPETE [2015-UID/NEU/04539/2013]
  4. FCT-Foundation for Science and Technology
  5. National Institutes of Health [P30-AG-028718, RO1-AG-033761]
  6. Fundação para a Ciência e a Tecnologia [PTDC/SAU-OSM/104124/2008, SFRH/BPD/26837/2006] Funding Source: FCT

向作者/读者索取更多资源

Type 2 diabetes mellitus is a complex metabolic disease, and cardiovascular disease is a leading complication of diabetes. Epicardial adipose tissue surrounding the heart displays biochemical, thermogenic, and cardio protective properties. However, the metabolic cross-talk between epicardial fat and the myocardium is largely unknown. This study sought to understand epicardial adipose tissue metabolism from heart failure patients with or without diabetes. We aimed to unravel possible differences in glucose and lipid metabolism between human epicardial and subcutaneous adipocytes and elucidate the potential underlying mechanisms involved in heart failure. Insulin-stimulated [C-14]glucose uptake and isoproterenol-stimulated lipolysis were measured in isolated epicardial and subcutaneous adipocytes. The expression of genes involved in glucose and lipid metabolism was analyzed by reverse transcription-polymerase chain reaction in adipocytes. In addition, epicardial and subcutaneous fatty acid composition was analyzed by high-resolution proton nuclear magnetic resonance spectroscopy. The difference between basal and insulin conditions in glucose uptake was significantly decreased (P = 0.006) in epicardial compared with subcutaneous adipocytes. Moreover, a significant (P < 0.001) decrease in the isoproterenol-stimulated lipolysis was also observed when the two fat depots were compared, and it was strongly correlated with lipolysis, lipid storage, and inflammation-related gene expression. Moreover, the fatty acid composition of these tissues was significantly altered by diabetes. These results emphasize potential metabolic differences between both fat depots in the presence of heart failure and highlight epicardial fat as a possible therapeutic target in situ in the cardiac microenvironment.

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