期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 48, 期 -, 页码 1-7出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2018.08.001
关键词
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资金
- Japan Science and Technology Agency (JST) Core Research for Evolutional Science and Technology (CREST)
- JSPS KAKENHI [17H06348]
- [17E17344]
- [18K14334]
Protein functions are tightly regulated by their subcellular localization and dynamic alteration. Chemical proteomics offers convenience and efficiency for profiling protein features in a native context. In this review, we summarize the recent progress of subcellular-compartment-focused chemical proteomics which do not rely on organelle fractionation. Organelle-specific activity-based protein profiling (ABPP) and engineered ascorbate peroxidase (APEX) have been developed for proteome analysis within organelles and even sub organelles. In parallel, our lab designed organelle-localizable reactive molecules (ORM5) to selectively tag nuclear and mitochondrial proteins. ORMs-based proteomics is applicable to primary neurons and brain slices, as well as cultured cell lines. In addition, we invented a conditional proteomics approach to elucidate zinc homeostasis by labeling and identifying proteins localized in Zn2+-rich space of live cells.
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