4.7 Review

Targets for repair: detecting and quantifying DNA damage with fluorescence-based methodologies

期刊

CURRENT OPINION IN BIOTECHNOLOGY
卷 55, 期 -, 页码 30-35

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2018.08.001

关键词

-

资金

  1. National Institutes of Health [ES023813]

向作者/读者索取更多资源

Detection and characterization of DNA damage is essential for evaluating genotoxicity, monitoring DNA repair, developing biomarkers for exposures, and evaluating the efficacy of chemotherapies. These diverse applications for DNA damage measurements have spurred the continual development and refinement of methodologies for detecting, characterizing, and quantifying DNA damage from isolated DNA and in cells and tissues. Current damage detection methods cover a wide range of techniques from radiolabeling to mass spectrometry, and use of these techniques varies widely based on expense, expertise, and knowledge of adduct formation. More generalizable, easy-to-use methods for detecting and quantifying DNA damage are needed, and there has been an emergence of fluorescence-based methodologies to address this need. Developments in these fluorescence-based strategies are reviewed here.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据