期刊
CRITICAL REVIEWS IN BIOTECHNOLOGY
卷 39, 期 3, 页码 337-350出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/07388551.2019.1566202
关键词
Pullulanase; homologous and heterologous expression; protein engineering; thermostability; solubility; optimization
资金
- Canada Research Chair grant
Thermoduric pullulanases, acting as starch-debranching enzymes, are required in many industrial applications, mainly in the production of concentrated glucose, maltose, and fructose syrups. To date, however, a single pullulanase, from Bacillus acidopullulyticus, is available on the market for industrial purposes. This review is an investigation of the major advances as well as the major challenges being faced with regard to optimization of the production of extracellular thermoduric pullulanases either by their original hosts or by recombinant organisms. The critical aspects linked to industrial pullulanase production, which should always be considered, are emphasized, including those parameters influencing solubility, thermostability, and catalytic efficiency of the enzyme. This review provides new insights for improving the production of extracellular thermoduric pullulanases in the hope that such information may facilitate their commercial utilization and potentially be applied to the development of other industrially relevant enzymes.
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