4.7 Article

Synthetic anionic surfaces can replace microparticles in stimulating burst coagulation of blood plasma

期刊

COLLOIDS AND SURFACES B-BIOINTERFACES
卷 175, 期 -, 页码 596-605

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.11.066

关键词

Blood coagulation; Hydroxyapatite; Microparticles; Thromboelastography; Plasma enhanced chemical vapor deposition (PECVD); Thrombin; FXII; FXIa; Contact pathway; Contact osteogenesis

资金

  1. Natural Sciences and Engineering Research Council of Canada (NSERC) [262874-2009]
  2. Canadian Institutes for Health Research (CIHR) [MOP-133729]
  3. George Mason University
  4. Fonds de la Recherche Quebec Sante (Bourse de chercheur national/National Research Scientist award) [22341]
  5. CONACyT (Mexico)
  6. MEDITIS Program (NSERC and Ecole Polytechnique)
  7. CONICET (Argentina)
  8. ELAP (Canada) scholarships

向作者/读者索取更多资源

Biomaterials are frequently evaluated for pro-coagulant activity but usually in the presence of microparticles (MPs), cell-derived vesicles in blood plasma whose phospholipid surfaces allow coagulation factors to set up as functional assemblies. We tested the hypothesis that synthetic anionic surfaces can catalyze burst thrombin activation in human blood plasma in the absence of MPs. In a thromboelastography (TEG) assay with plastic sample cups and pins, recalcified human citrated platelet-poor plasma spontaneously burst-coagulated but with an unpredictable clotting time whereas plasma depleted of MPs by ultracentrifugation failed to coagulate. Coagulation of MP-depleted plasma was restored in a dose-dependent manner by glass microbeads, hydroxyapatite nanoparticles (HA NPs), and carboxylic acid-containing anionic nanocoatings of TEG cups and pins (coated by glow-discharge plasma-polymerized ethylene containing oxygen, L-PPE:O with 4.4 and 6.8 atomic % [COOH]). Glass beads lost their pro-coagulant activity in MP-depleted plasma after their surfaces were nano coated with hydrophobic plasma-polymerized hexamethyl disiloxane (PP-HMDSO). In FXII-depleted MP-depleted plasma, glass microbeads failed to induce coagulation, however, FXIa was sufficient to induce coagulation in a dose-dependent manner, with no effect of glass beads. These data suggest that anionic surfaces of crystalline, organic, and amorphous solid synthetic materials catalyze explosive thrombin generation in MP-depleted plasma by activating the FXII-dependent intrinsic contact pathway. The data also show that microparticles are pro coagulant surfaces whose activity has been largely overlooked in many coagulation studies to-date. These results suggest a possible mechanism by which anionic biomaterial surfaces induce bone healing by contact osteogenesis, through fibrin clot formation in the absence of platelet activation.

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