期刊
CHINESE CHEMICAL LETTERS
卷 30, 期 5, 页码 1017-1020出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2019.01.029
关键词
Aptamer; Aflatoxin B1; Fluorescence; Molecular beacons; Mycotoxin; Sensor
资金
- National Natural Science Foundation of China [21575153, 21435008, 21874146]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14030200]
- Key Research Program of the Chinese Academy of Sciences [KFZD-SW-203]
Aflatoxin B1 (AFB1) is a highly toxic mycotoxin, and rapid and sensitive detection of AFB1 is in demand for food safety and environmental analysis. Here we described a simple aptamer molecular beacon assay for rapid detection of aflatoxin B1 (AFB1) by using an aptamer with a fluorescein (FAM) label at the 5' end and a fluorescence quencher (black hole quencher 1, BHQ1) at the 3' end. In the presence of AFB1, the aptamer probe bound with AFB1 and induced a hairpin structure, drawing FAM and BHQ1 into close proximity and leading to fluorescence quenching. This assay allowed for a detection limit of 3.9 nmol/L and a dynamic range from 3.9 nmol/L, to 4 mu mol/L. Specificity test showed other mycotoxins including ochratoxin A, ochratoxin B, fumonisin B1, fumonisin B2, and zearalenone had negligible influence on detection of AFB1. AFB1 spiked in diluted liquor wine, methanol, or corn flour samples was successfully detected by using this aptamer probe, and the assay showed potential for real sample analysis. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据