期刊
CHEMOSPHERE
卷 216, 期 -, 页码 524-532出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2018.10.140
关键词
Decabromodiphenyl ethane (DBDPE); Cell membrane damage; Membrane interaction; Molecular dynamic simulations
资金
- National Natural Science Foundation of China [31000017, 21207056]
- State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences [KF2015-17]
- Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the University of Gansu Province, Gansu University of Chinese Medicines [zzy-2016-01]
- Fundamental Research Funds for the Central Universities [lzujbky-2017-200, lzujbky-2017-kb05, LZU-JZH1914]
- Gansu Provincial Administration of traditional Chinese Medicine [GZK-2017-63]
- Lanzhou talent innovation and entrepreneurship technology program [2016-RC-19]
Decabromodiphenyl ethane (DBDPE) is widely used in industry as an alternative to the decabromodiphenyl ether (BDEs). The large-scale use of DBDPE could lead to rapid growth of the human accumulation level of DBDPE. However, the biophysics of accumulation of DBDPE in cell membranes, as one of determinants of DBDPE metabolism is not clear. In the present study, detailed observations of cell lactate dehydrogenase (LDH) and reactive oxygen species (ROS) levels measurements proved that the DBDPE exposure to cell could result in significant cell membrane damage by concentration-dependent manners. The fluorescence anisotropy analysis supported the evidence that high concentration DBDPE bound decreased membrane fluidity significantly. Besides it, a detailed molecular dynamic (MD) simulation was approached to investigate the effects of DBDPE on the DPPC (dipalmitoyl phosphatidylcholine) phospholipid bilayer, which was constructed as the model of cell membrane. The molecular dynamic simulation revealed that DBDPE molecules can easily enter the membrane from the aqueous phase. Under the concentration of a threshold, the DBDPE molecules tended to aggregate inside the DPPC bilayer and caused pore formation. The bound of high concentration of DBDPE could result in significant variations in DPPC bilayer with a less dense, more disorder and rougher layer. The knowledge about DBDPE5 interactions with lipid membranes is fundamentally essential to understand the in vivo process of DBDPE and the physical basis for the toxicity of DBDPE in cell membranes. (C) 2018 Elsevier Ltd. All rights reserved.
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