期刊
CELL DEATH AND DIFFERENTIATION
卷 26, 期 10, 页码 2115-2124出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41418-019-0280-2
关键词
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资金
- China National Basic Research Program [JFYS 2016YFA0100203]
- Doctoral Scientific Research Foundation of 2017 [Z109021715]
- Program of National Natural Science Foundation of China [31572399, 31272518]
- China Postdoctoral Science Foundation [K3080217064]
In vitro induction of functional haploid cells from embryonic stem cells (ESCs) has been reported by several groups. However, these reports either involve complex induction process with undefined induction factors or show low-induction efficiency. Here, we report complete meiosis in vitro from ESCs with defined induction factors. ESCs were first induced into primordial germ cell-like cells, which were further induced into male germline cells, including spermatogonial stem cell-like cells (SSCLCs) and spermatid-like cells. Importantly, the obtained SSCLCs were functional as infertile male mice sired healthy offspring via SSCLC transplantation. Further, we found that eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) was essential for spermatogenesis. Eif2s3y-overexpressing ESCs showed enhanced spermatogenesis in vitro, as demonstrated by higher expression levels of SSC-specific markers during SSCLC induction process, improved reproductive ability recovery of infertile male mice, and increased efficiency of haploid cell induction. Our work provides a convenient and efficient approach to obtain functional male germline cells.
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