Does Drug-Eluting Bead TACE Enhance the Local Effect of IRE? Imaging and Histopathological Evaluation in a Porcine Model

ObjectivesWe conducted an in vivo trial on swine to compare the ablation volumes of irreversible electroporation (IRE) followed by drug-eluting beads transarterial chemoembolization (DEB-TACE) versus IRE only.Materials and MethodsNine swine underwent CT-guided IRE in one liver lobe and IRE immediately followed by DEB-TACE in a different liver lobe. For DEB-TACE, 100-300 mu m beads (DC-Beads((R))) were loaded with 50mg doxorubicin. For IRE, the NanoKnife((R)) was used employing two electrodes according to the vendor's protocol. Imaging follow-up was performed including CT-based lesion volume assessment using contrast-enhanced CT (venous phase) on days 1, 3, and 7 after the procedure. Three animals were killed for histopathological analysis after each follow-up.ResultsAblation volumes in CT in the IRE+DEB-TACE group were 15.410.5ml on day 1, 8.7 +/- 5.6ml on day 3, and 1.6 +/- 0.7ml on day 7. In the IRE group, the corresponding values were 5.2 +/- 5.2ml on day 1, 1.0 +/- 1.2ml on day 3, and 0.1 +/- 0.1ml on day 7. On day 1 and day 3, ablation volumes of IRE+TACE group were significantly larger than in the IRE group (p<0.05). 96% of beads were depicted in or around ablative lesions. 69% of these beads were found in the surrounding hemorrhagic infiltration and 31% within the ablative lesion itself.Conclusionsp id=ParCombination of IRE immediately followed by DEB-TACE resulted in larger ablation volumes compared to IRE alone, suggesting that local efficacy of IRE can be enhanced by post-IRE DEB-TACE.