期刊
CARBOHYDRATE POLYMERS
卷 207, 期 -, 页码 729-736出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.12.047
关键词
Exochitinase; Thermophilicity; 85% deacetylated chitosan; Chitooligosaccharides; Anti-oxidant activity
资金
- National Natural Science Foundation of China [31570046]
- Priority Academic Development Program of Jiangsu Higher Education Institutions
- Scientific Innovation
ChiEn3 from Coprinopsis cinerea was characterized as an exo-acting chitinase with a processivity. ChiEn3 hydrolyzed only soluble chitin and exhibited a hyperhydrolytic activity toward 85% deacetylated chitosan which was 33.6-fold higher than its hydrolytic activity toward glycol chitin. Its maximum hydrolytic activity was observed at 60 degrees C and retained 66.2% of hydrolytic activity after 60 min incubation at 60 degrees C. Commercial 85% deacetylated chitosan was degraded by ChiEn3 to a series of COSs with a DP of 2-20 in which COSs with a DP of 3-6 were dominant, whereas, lab-prepared chitosan (F-A = 0.65) was degraded by ChiEn3 to COSs with a DP of 2-10 in which the AA dimer was dominant. DPPH-radical-scavenging activity of ChiEn3-digested products of 85% deacetylated chitosan was 3.32-fold higher than that of undigested 85% deacetylated chitosan. Therefore, ChiEn3 shows a valuable advantage for application to the preparation of COSs from commercial 85% deacetylated chitosan.
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