期刊
CANCER SCIENCE
卷 110, 期 4, 页码 1464-1479出版社
WILEY
DOI: 10.1111/cas.13968
关键词
clinical sequencing; junction capture method; molecular profiling; personalized medicine; Todai OncoPanel
类别
资金
- Japan Agency for Medical Research and Development [17kk0205003h0002, JP17am0001001, JP17ck0106252, JP17cm0106502]
- Sysmex Corporation
- Eisai
Tumor molecular profiling is becoming a standard of care for patients with cancer, but the optimal platform for cancer sequencing remains undetermined. We established a comprehensive assay, the Todai OncoPanel (TOP), which consists of DNA and RNA hybridization capture-based next-generation sequencing panels. A novel method for target enrichment, named the junction capture method, was developed for the RNA panel to accurately and cost-effectively detect 365 fusion genes as well as aberrantly spliced transcripts. The TOP RNA panel can also measure the expression profiles of an additional 109 genes. The TOP DNA panel was developed to detect single nucleotide variants and insertions/deletions for 464 genes, to calculate tumor mutation burden and microsatellite instability status, and to infer chromosomal copy number. Clinically relevant somatic mutations were identified in 32.2% (59/183) of patients by prospective TOP testing, signifying the clinical utility of TOP for providing personalized medicine to cancer patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据