期刊
BMC PLANT BIOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12870-019-1715-0
关键词
Gene editing; Heterosis; Thermo-sensitive genic male sterility (TGMS); TMS5 gene; Two-line hybrid rice system
资金
- National Key Research and Development Program of China [2016YFD0101801, 2017YFD0100300]
- National S AMP
- T Major Project of China [2016ZX08001006, 2016ZX08001001]
- Central Level, Non-Profit, Scientific Research Institutes Basic RAMP
- D Operations Special Fund [Y2017PT46, 2017RG002-1]
Background: Two-line hybrid rice with high yield potential is increasingly popular and the photo- and temperature-sensitive male sterile line is one of the basic components for two-line hybrid rice breeding. The development of male sterile lines through conventional breeding is a lengthy and laborious process, whereas developing thermo-sensitive genic male sterile (TGMS) lines for two-line hybrid breeding by editing a temperature-sensitivity gene by CRISPR/Cas9 is efficient and convenient. Results: Here, thermo-sensitive genic male sterility (TGMS) was induced by employing the CRISPR/Cas9 gene editing technology to modify the gene TMS5. Two TGMS mutants, tms5-1 and tms5-2, both lacking any residual T-DNA, were generated in the indica rice cultivar Zhongjiazao17 (cv. YK17) background. When grown at a sub-optimal temperature (22 degrees C), both mutants produced viable pollen and successfully produced grain through self-fertilization, but at temperatures 24 and 26 degrees C, their pollen was sterile and no grain was set. F-1 hybrids derived from the crosses between YK17S (tms5-1) and three different restorer lines outperformed both parental lines with respect to grain yield and related traits. Conclusion: The YK17S generated by CRISPR/Cas9 system was proved to be a new TGMS line with superior yield potential and can be widely utilized in two-line hybrid breeding of indica rice.
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