期刊
BIOSENSORS & BIOELECTRONICS
卷 126, 期 -, 页码 455-462出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.11.020
关键词
Immunosensor; Label- and amplification-free; Photoelectric effect; Au/TiO2; Layer-by-layer; Sandwich
类别
资金
- Ministry of Science and Technology, Taiwan [104-2113-M-005-011-MY3, 106-2627-M-005-001-, 107-2113-M-005-008-, 107-2627-M-005-001-]
A label- and amplification-free photoelectric immunosensor based on well-defined layer-by-layer sandwich-structured AuNP/TNW/AuNP composite is proposed for direct and ultrasensitive detection of alpha-fetoprotein (AFP). The AuNP/TNW/AuNP composite is produced by assembling an Au nanoparticle underlayer and anatase TiO2 nanowires (TNW) onto the FTO substrate, followed by decorating Au nanoparticles onto the TNW surface, through a simple sputtering and hydrothermal process. The resulting AuNP/TNW/AuNP electrode exhibits a 14-fold and 2-fold enhancement in photocurrent density under simulated sunlight compared with that of bare TNW and AuNP/TNW, respectively, which benefits from the SPR-induced photoabsorption increment and charge separation improvement in Au nanoparticle and interfacial charge transfer promotion at the TiO2/substrate interface in the Au underlayer. As a proof of concept, the AFP antigen can be quantitatively detected by the proposed AuNP/TNW/AuNP coupled with anti-AFP through the analysis of the photocurrent change. This novel AFP photoelectric immunosensor exhibited sensitive detection of AFP with an ultrahigh sensitivity of 0.001 ng mL(-1) and good specific selectivity. Moreover, the practical determination of AFP in human serum is also investigated, demonstrating its applicability and potential attraction for clinical tests and disease diagnosis.
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