期刊
BIOSENSORS & BIOELECTRONICS
卷 126, 期 -, 页码 151-159出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.10.033
关键词
Immunoassay; Dual-signal readout; Hybridization chain reaction; Microcystin-LR
类别
资金
- National Natural Science Foundation of China [21475047, 21705051, 21874048]
- Science and Technology Planning Project of Guangdong Province [2016B030303010]
- Scientific Foundation of Guangdong Province [2017A030313077]
- National Key Research and Development Program of China [SQ2017YFC160089]
- Program for the Top Young Innovative Talents of Guangdong Province [2016TQ03N305]
- Science and Technology Innovation Project of Guangdong University Students [pdjhb0086]
- Foundation for High-level Talents in South China Agricultural University
- Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences [1630122017011, 1630122017009]
Enzyme-based electrochemical biosensors are widely used in immunoassays, but the intrinsic disadvantages of enzymes including instability or sensitivity to temperature and pH should be considered. Herein, an enzyme-free and dual-signal readout immunoassay was established to detect microcystin-LR (MC-LR) sensitively and selectively. Firstly, the microplate was modified with gold nanoparticles-decorated-carbon nanotubes (AuNP-CNT) to immobilize sufficient antigens by the high surface area of CNT and high affinity of AuNP. Then, silver nanoparticles were decorated on gold nanorods to form corn-like AgNP/AuNR composite and then capture secondary antibody and initiator DNA strand. After hybridization chain reaction, long double helix DNA strands can be formed on AgNP/AuNR to germinate copper nanoparticles. A dual-signal readout from the current responses of both silver and copper ions was obtained by using differential pulse stripping voltammetry with the aid of acid treatment, By using a competitive immunoreaction, MC-LR can be detected in a linear range from 0.005 mu g/L to 20 mu g/L with a lower detection limit of 2.8 mu g/L. The reproducibility, stability and specificity were all acceptable, indicating its promising application in environment monitoring and sensitive electrochemical detection for other analytes.
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