期刊
BIORESOURCE TECHNOLOGY
卷 273, 期 -, 页码 269-276出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.11.016
关键词
Co-fermentation; Ethanol; E. coli; Biomass; Xylose
资金
- US Department of Energy's Office of International Affairs [DE-PI0000031]
Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coil (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/ enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24.9 +/- 0.8 g.L-1 (70% of the theoretical yield) in < 30 h. Ethanol titer increased to 48.6 +/- 1.04 g.L-1 (yield, 0.45 g.g(-1) sugars) at a solids content of 20% and the highest rate of xylose consumption was 1.58 +/- 0.21 g.L-1.L-1. This study demonstrates the potential of a co-culture of strain LW419a and yeast to rapidly ferment all the sugars in pretreated biomass slurries to ethanol at their respective highest rates.
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