期刊
BIORESOURCE TECHNOLOGY
卷 276, 期 -, 页码 42-50出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.12.088
关键词
Domestic wastewater; FPase; Membrane separation; Stirred tank reactor (STR); Bubble column bioreactor (BCR)
资金
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- National Council of Technological and Scientific Development (CNPq), Brazil
The production of cellulases using domestic wastewater as an alternative culture medium and reducing the pollutant charge of the resultant effluents were assessed for the first time in this study. Cellulase production was carried out in a bubble column, column-packed bed and stirred tank reactors by Trichoderma harzianum. Maximum cellulase activity and productivity of 31 UFP/mL and 645 UFP/mL. h, respectively were achieved in the bubble column bioreactor system without immobilization. The fermented broth was microfiltrated and ultrafiltrated, leading to a cellulase recovery of 73.5% using a 30 kDa membrane and resulting in a 4.23-fold activity concentration. Chemical oxygen demand and nitrogen concentration were reduced 81.37% and 52.9%, respectively, showing great promise in producing cellulases using domestic wastewater with concomitant development of a medium- to- high added- value process and reduced environmental impact. These results contribute to the development of sustainable bioprocesses approaching a biorefinery concept.
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