期刊
BIOORGANIC CHEMISTRY
卷 93, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2019.03.040
关键词
Glyphosate oxidoreductase; N-phosphonomethylglycine biodegradation; Phosphorus-nitrogen bond cleavage; Psychrotolerant yeasts
资金
- Polish Ministry of Science and Higher Education
Solicoccozyma terricola M 3.1.4., the yeast strain isolated from soil sample from blueberry cultivation in Miedzyrzec Podlaski in Poland, is capable to split of phosphorus to nitrogen and nitrogen to carbon bonds in N-phosphonomethylglycine (PMG, glyphosate). The biodegradation process proceeds in the phosphate-independent manner. It is the first example of a psychrotolerant yeast strain able to degrade PMG via C-N bond cleavage accompanied by AMPA formation and not like in most microorganisms via C-P bond disruption followed by the sarcosine pathway. Glyphosate oxidoreductase (GOX) type activity was detected in cell-free extracts prepared from S. terricola M 3.1.4. pregrown on 4mM PMG as a sole phosphorus and nitrogen source in cultivation medium.
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