期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 27, 期 6, 页码 1087-1098出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2019.02.014
关键词
Indoleamine 2,3-dioxygenase 1 (IDO1); Tryptophan 2,3-dioxygenase (TDO); 1H-Indazoles; Dual inhibitor; Cancer immunotherapy; Antitumor activity
资金
- Youth Fund of Sichuan Science and Technology Department [2017JQ0023]
- Chengdu Technology Innovation Research Project [2018-YFYF-00195-SN]
- Found of Sichuan Education Department [18TD0023]
- Sichuan Science and Technology Innovation Seedling Project [2018082]
- National Natural Science Foundation of China [21861142007, 21672207]
- Chinese Academy of Sciences President's International Fellowship Initiative [2015PB049]
- Open Research Subject of Health
- Innovation Fund of Undergraduate and Post Graduate from Xihua University
Indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) are constitutively overexpressed in many types of cancer cells and exert important immunosuppressive functions. In this article, a series of 4,6-substituted-1H-indazole derivatives were synthesized and evaluated the inhibitory activities against IDO1 and TDO, as well as their structure-activity relationships (SARs). Among these, compound 35 displayed the most IDO1 inhibitory potency with an IC50 value of 0.74 mu M in an enzymatic assay and 1.37 mu M in HeLa cells. Quantitative analysis of the Western blot results indicated that 35 significantly decreased the INF gamma-induced IDO1 expression in a concentration-dependent manner. In addition, 35 showed promising TDO inhibition with an IC50 value of 2.93 mu M in the enzymatic assay and 7.54 mu M in A172 cells. Moreover, compound 35 exhibited in vivo antitumor activity in the CT26 xenograft model. These findings suggest that 1H-indazole derivative 35 is a potent IDO1/TDO dual inhibitor, and has the potential to be developed for IDO1/TDO-related cancer treatment.
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