期刊
BIOCHEMISTRY
卷 58, 期 12, 页码 1596-1615出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b00007
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资金
- National Institutes of Health [AI119567]
- National Science Foundation [CHE-1709564]
- Arthur Cope Foundation
Menaquinones (MKs) are essential for electron transport in prokaryotes, and importantly, partially saturated MKs represent a novel virulence factor. However, little is known regarding how the degree of saturation in the isoprenyl side chain influences conformation or quinone redox potential. MenJ is an enzyme that selectively reduces the second isoprene unit on MK-9 and is contextually essential for the survival of Mycobacterium tuberculosis in J774A.1 macrophage-like cells, suggesting that MenJ may be a conditional drug target for pathogenic mycobacteria. Therefore, fundamental information about the properties of this system is important, and we synthesized the simplest MKs, unsaturated MK-1 and the saturated analogue, MK-1(H-2). Using two-dimensional nuclear magnetic resonance spectroscopy, we established that MK-1 and MK-1(H-2) adopted similar folded-extended conformations (i.e., the isoprenyl side chain folds upward) in each solvent examined but the folded-extended conformations differed slightly between organic solvents. Saturation of the isoprenyl side chain slightly altered the MK-1 analogue conformation in each solvent. We used molecular mechanics to illustrate the MK-1 analogue conformations. The measured quinone redox potentials of MK-1 and MK-1(H-2) differed between organic solvents (presumably due to differences in dielectric constants), and remarkably, an similar to 20 mV semiquinone redox potential difference was observed between MK-1 and MK-1(H-2) in pyridine, acetonitrile, and dimethyl sulfoxide, demonstrating that the degree of saturation in the isoprenyl side chain of MK-1 influences the quinone redox potential. Finally, MK-1 and MK-1(H-2) interacted with Langmuir phospholipid monolayers and Aerosol-OT reverse micelle (RM) model membrane interfaces, where MK-1 adopted a slightly different folded conformation within the RM model membrane interface.
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