期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 512, 期 4, 页码 729-735出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2019.03.119
关键词
Metabolomics; Lipidomics; Exosomes; Mesenchymal stem cells
资金
- UC Office of the President's Multi-campus Research Program Grant [MRP-17-454909]
- STAIR Grant
- STAIR-Plus Grant CTSC Rapid Translational Grant [UL1-TR001860]
- T32 Cardio (NIH) [T32-HL086350]
- Denny & Jeanene Dickenson Fund
- NIH Transformative [R01GM099688]
- NSF [GROW 201111600, GRFP 2011116000]
- NIH [T32-GM008799]
- [U2C ES030158]
Mesenchymal stem cell (MSC) based therapies are currently being evaluated as a putative therapeutic in numerous human clinical trials. Recent reports have established that exosomes mediate much of the therapeutic properties of MSCs. Exosomes are nanovesicles which mediate intercellular communication, transmitting signals between cells which regulate a diverse range of biological processes. MSC-derived exosomes are packaged with numerous types of proteins and RNAs, however, their metabolomic and lipidomic profiles to date have not been well characterized. We previously reported that MSCs, in response to priming culture conditions that mimic the in vivo microenvironmental niche, substantially modulate cellular signaling and significantly increase the secretion of exosomes. Here we report that MSCs exposed to such priming conditions undergo glycolytic reprogramming, which homogenizes MSCs' metabolomic profile. In addition, we establish that exosomes derive from primed MSCs are packaged with numerous metabolites that have been directly associated with immunomodulation, including M2 macrophage polarization and regulatory T lymphocyte induction. (C) 2019 The Authors. Published by Elsevier Inc.
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