期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 511, 期 4, 页码 725-731出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2019.02.141
关键词
TXNIP; NLRP3; Monosodium urate; NF-kappa B; Reactive oxygen species
资金
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [2016R1D1A1B03933687]
- National Research Foundation of Korea [2016R1D1A1B03933687] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Objective: The aim of this study was to assess the role of thioredoxin-interacting protein (TXNIP) in nuclear factor-kappa B (NF-kappa B) signaling and the interaction between TXNIP and NOD-like receptor protein 3 (NLRP3) in activation of the NLRP3 inflammasome in monosodium urate (MSU)-induced inflammation. Methods: Interleukin-1 beta (IL-1 beta), IL-18, caspase-1, phospho-I kappa B alpha (pI kappa B alpha), phospho-NF-kappa B, (pNF-kappa B), and TXNIP in U937 macrophage-like cells treated with MSU crystals were analyzed using western blotting and real-time polymerase chain reaction (RT-PCR). Expression of these molecules was also assessed in U937 macrophages transfected with TXNIP siRNA and treated with antioxidants. Results: U937 macrophages treated with MSU crystals showed increased expression of IL-1 beta, IL-18, caspase-1, and TXNIP and activation of NF-kappa B signaling, which were strongly inhibited by addition of antioxidants or transfection with TXNIP siRNA. Intracellular translocation of TXNIP from the nucleus to mitochondria was observed in cells treated with MSU crystals. And quercetin and ascorbic acid suppressed translocation of TXNIP. Binding between TXNIP and NLRP3 under oxidative stress caused by MSU crystals was observed and was blocked by quercetin or ascorbic acid. Conclusion: This study showed that activation of MSU-induced NLRP3 inflammasome requires TXNIP-mediated NF-kappa B signaling pathway and intracellular TXNIP shifting. (C) 2019 Published by Elsevier Inc.
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