期刊
AUTOPHAGY
卷 15, 期 5, 页码 922-923出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2019.1582952
关键词
Autophagosome formation; CRISPR; DedA family; GFP-LC3-RFP reporter; lipid droplet; VMP1; VTT domain
类别
资金
- Japan Science and Technology Agency [JPMJER1702]
Macroautophagy/autophagy requires many autophagy-related (ATG) proteins. Most of the ATG genes were identified by genetic screening using simple model organisms. Recently, we performed a forward genetic screen in mammalian cells using the CRISPR-Cas9 system and our autophagic flux reporter GFP-LC3-RFP. One of the identified proteins was TMEM41B, an ER-localized multi-spanning membrane protein. TMEM41B has a characteristic transmembrane domain (the VTT domain), which is also found in VMP1, another protein involved in autophagy. Our results show that TMEM41B and VMP1 are physically and functionally associated.
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