期刊
ANIMAL REPRODUCTION SCIENCE
卷 201, 期 -, 页码 63-70出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2018.12.010
关键词
Neuropeptides; Ovarian development
资金
- Natural Science Foundation of China [41476130]
- Shanghai University Youth Teacher Training Program [ZZSHOU16007]
- Shanghai Sailing Program [17YF1408000]
- Special Fund for the Development of Science and Technology of Shanghai Ocean University [A2-0203-17-100201]
Neuropeptides, important messenger molecules, regulate various physiological processes, such as Neuropeptides growth, development, and reproduction. In the present study, cDNA libraries from brains of E. Ovarian development sinensis were constructed and sequenced using the Illumina technique for transcript analysis and neuropeptides discovery. There were 233,887 transcripts assembled for 194,286 unigenes. According to the annotations of NCBI non-redundant protein (NR) database, 2487 (11.31%) unigenes were annotated successfully. In total, 1273 transcripts were assigned to the signal transduction mechanisms category using KOG analysis. The results of KEGG indicate signal transduction and translation pathways were the dominant and enriched signal pathways. Additionally, results indicated C2H2 was the main transcription factor (TF) family. Analysis of the assembled transcripts indicated there were 22 neuropeptide transcripts, such as allatostatin, crustacean female sex hormone, crustacean hyperglycemic hormone, diuretic hormone 31, and eclosion hormone. The detection of these neuropeptides provide for a basic understanding for future study of functions in development, reproduction, and sexual maturation in crustaceans.
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