期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 18, 页码 5910-5914出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201812892
关键词
astrocytes; immunochemistry; lipids; mass spectrometry; neurons
资金
- National Institute of Mental Health [1U01MH109062]
- National Institute on Drug Abuse Award [P30DA018310]
- National Human Genome Research Institute [RM1HG010023]
- National Science Foundation Graduate Research Fellowship Program
- Springborn Fellowships
Transcriptomics characterizes cells based on their potential molecular repertoire whereas direct mass spectrometry (MS) provides information on the actual compounds present within cells. Single-cell matrix-assisted laser desorption/ionization (MALDI) MS can measure the chemical contents of individual cells but spectra are difficult to correlate to conventional cell types, limiting the metabolic information obtained. We present a protocol that combines MALDI-MS with immunocytochemistry to assay over a thousand individual rat brain cells. The approach entwines the wealth of knowledge obtained by immunocytochemical profiling with mass spectral information on the predominant lipids within each cell. While many lipid species showed a high degree of similarity between neurons and astrocytes, seventeen significantly differed between the two cell types, including four phosphatidylethanolamines elevated in astrocytes and nine phosphatidylcholines in neurons.
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