期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 20, 页码 6590-6594出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201901320
关键词
bioimaging; DNA-based sensor; nucleic acids; signaling molecules; synthetic cofactor
资金
- National Natural Science Foundation of China [21725503, 21575038, 21675044, 21505120]
- Young Top-notch Talent for Ten Thousand Talent Program
- Fundamental Research Funds for the Central Universities
The comprehensive understanding of the mechanisms underlying the interaction of cells with their membrane microenvironment is of great value for fundamental biological research; however, tracking biomolecules on cell surfaces with high temporal and spatial resolution remains a challenge. Herein, a modular strategy is presented for the construction of cell surface DNA-based sensors by engineering DNA motifs and synthetic cofactors. In this strategy, a stimuli-reactive organic molecule is employed as the cofactor for the DNA motif, and the self-assembly of them forms a FRET-based holo DNA-based sensor. With the use of the DNA-based sensors, the versatility of this modular strategy has been demonstrated in the ratiometric imaging of the cellular extrusion process of endogenous signaling molecules, including sulfur dioxide derivatives and nitric oxide.
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