期刊
ANALYTICAL CHEMISTRY
卷 91, 期 8, 页码 5028-5035出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b05084
关键词
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资金
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-SC0013978]
- University of Missouri's Gus T. Ridgel Fellowship
- George Washington Carver Fellowship
- National Science Foundation Plant Genome Program [IoS-1734145]
- U.S. Department of Energy (DOE) [DE-SC0013978] Funding Source: U.S. Department of Energy (DOE)
Mass spectrometry (MS) is an indispensable analytical tool to capture the array of metabolites within complex biological systems. However, conventional MS-based metabolomic workflows require extensive sample processing and separation resulting in limited throughput and potential alteration of the native molecular states in these systems. Ambient ionization methods, capable of sampling directly from tissues, circumvent some of these issues but require high-performance MS to resolve the molecular complexity within these samples. Here, we demonstrate a unique combination of laser ablation electrospray ionization (LAESI) coupled with a 21 tesla Fourier transform ion cyclotron resonance (21T-FTICR) for direct MS analysis and imaging applications. This analytical platform provides isotopic fine structure information directly from biological tissues, enabling the rapid assignment of molecular formulas and delivering a higher degree of confidence for molecular identification.
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