期刊
ANALYTICAL BIOCHEMISTRY
卷 566, 期 -, 页码 10-12出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2018.10.029
关键词
Quorum quenching; High-throughput; AHL acylase; Fluorescamine; Fluorescence
资金
- Maurice Wilkins Centre for Molecular Biodiscovery, New Zealand
- Ministry of Business, Innovation and Employment, New Zealand
- University of Otago Doctoral Scholarship, New Zealand
N-acyl-L-homoserine lactone (AHL) acylases are a well-known group of enzymes that disrupt quorum sensing in Gram-negative bacteria by degrading AHL signalling molecules. This degradation of signalling molecules (termed 'quorum quenching') has potential uses in the prevention or reduction of biofilm formation and/or bacterial infections. Therefore, there is a great deal of interest in the identification and characterisation of quorum quenching enzymes. Here, we present an optimised fluorescamine-based assay for the detection of AHL acylase activity and demonstrate it can be used in a high-throughput screening format.
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