期刊
ANALYTICA CHIMICA ACTA
卷 1064, 期 -, 页码 87-93出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.03.004
关键词
Ratiometric fluorescence; Probe; Hypochlorous acid/hypochlorite; New mechanism; Cell image
资金
- National Key Research and Development Program of China [2017YFA0104604]
- Natural Science Foundation of Shandong Province [ZR2018MB042]
- National Natural Science Foundation of China [81502948, 31741083, 31871407]
A ratiometric fluorescence probe (named ZOC) for the fast detection of HClO/ClO- was constructed by coumarin (donor) and pyridinium (acceptor) based on Forster resonance energy transfer (FRET) and intramolecular charge transfer (Icr) platform. ZOC possessed red emission signal (610 nm), large Stocks shift (190 nm), high energy transfer efficiency (95.3%), high selectivity and sensitivity, low detection limit (25 nM), wider detection range (from 25 nM to 30 mu M), rapid response (within 13 S), and good biocompatibility. It was very interesting that the recognition mechanism involved a new organic reaction in which olefin double bond reacted first with HClO/ClO - regioselectively, followed by cyclization. ZOC was successfully used to the real time detection of endogenous HClO/ClO- in RAW 264.7 cells. (C) 2019 Elsevier B.V. All rights reserved.
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