期刊
ANALYTICA CHIMICA ACTA
卷 1050, 期 -, 页码 51-59出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.11.004
关键词
Acetamiprid; Aptasensor; Copper-centered metal-organic frameworks; Biosensor
资金
- Shandong Provincial Natural Science Foundation, China [ZR2017MB063]
- National Natural Science Foundation of China (NSFC) [31370090]
- Project of Key R&D of Shandong Province in China [2015GSF121006]
In present work, a versatile signal-on electrochemical aptasensor with ultra-sensitivity and high selectivity for detecting acetamiprid residues has been successfully constructed. Electrochemistry behaviors of as-synthesized copper-centered metal-organic frameworks (CuMOF) on various electrodes were investigated in details. The results indicated that CuMOF exhibited well-behaved redox events. Thus, we used Au-CuMOF as signaling element to label probe DNA (pDNA). The gold nanoparticles-reduced graphene oxide (Au-rGO) has a high specific surface area and excellent conductivity, which was utilized to immobilize complementary strand (cDNA). In the presence of acetamiprid, Au-CuMOF-labeled pDNA would hybridize with the exposed cDNA, allowing CuMOF to approach the electrode and produce a sensitive signaling current. Such a signal-on method does not suffer from the drawbacks of signal-off methods. The linear range of this proposed electrochemical aptasensor was 0.1 pM-10.0 nM and the detection limit was as low as 2.9 fM. This platform exhibited wonderful selectivity, stability, and repeatability, and was successfully applied to detect acetamiprid residues in tea samples exhibiting enormous practical application potential. (C) 2018 Elsevier B.V. All rights reserved.
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