期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 38, 期 4, 页码 552-558出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b14-00700
关键词
3T3-L1 cell; sinensetin; adipogenesis; lipolysis; cAMP
资金
- National Research Foundation of Korea (NRF) by the Ministry of Education [2013R1A1A2A10004415]
- National Research Foundation of Korea [2013R1A1A2A10004415] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Sinensetin is a rare polymethoxylated flavone (PMF) found in certain citrus fruits. In this study, we investigated the effects of sinensetin on lipid metabolism in 3T3-L1 cells. Sinensetin promoted adipogenesis in 3T3-L1 preadipocytes growing in incomplete differentiation medium, which did not contain 3-isobutyl-1-methylxanthine. Sinensetin up-regulated expression of the adipogenic transcription factors peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein (C/EBP) alpha, and sterol regulatory element-binding protein 1c. It also potentiated expression of C/EBP beta and activation of cAMP-responsive element-binding protein. Sinensetin enhanced activation of protein kinase A and increased intracellular cAMP levels in 3T3-L1 preadipocytes. In mature 3T3-L1 adipocytes, sinensetin stimulated lipolysis via a cAMP pathway. Taken together, these results suggest that sinensetin enhances adipogenesis and lipolysis by increasing cAMP levels in adipocytes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据