期刊
AGING-US
卷 11, 期 5, 页码 1342-1355出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.101835
关键词
beta cells; miR-127; cell viability; insulin secretion; EVs
资金
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX17_1238]
- National Natural Science Foundation of China [81530026, 81600640]
MicroRNAs (miRNAs) have been implicated in beta cells dysfunction. Previous studies indicated that miR-127 was specifically abundant in beta cells and one of its target genes, Kif3b, promoted cell proliferation. However, the impact of the miR-127-Kif3b axis on beta cells remains unknown. In this study, we revealed that miR-127 level was declined both in islets from the mice with a high-fat diet and in MIN6 cells with elevated glucose treatment. The elevated level of miR-127 attenuated beta cell proliferation by repressing Kif3b expression without affecting apoptosis and cell cycle, and it dampened insulin secretion. Moreover, beta cell-derived miR-127 could also affect the islet endothelial cell-line, MS1, in vitro via the transfer of extracellular vesicles (EVs). Treating MS1 cells with the EVs secreted by MIN6 cells exhibited a higher ability in cell migration and tube formation. However, this effect was abolished by the miR-127 inhibitor co-cultured with EVs-treated MS1 cells. Thus, we define that miR-127 is a crucial regulator of insulin secretion and cell proliferation in pancreatic beta cells as well as a potential functional regulation factor in islet endothelial cells.
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