期刊
ADVANCED MATERIALS
卷 31, 期 18, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201808021
关键词
biofuels; butanol tolerance; conjugated oligoelectrolytes; membrane integrity; membrane stability
类别
资金
- NTU start up grant [M4081984.120]
- Singapore Ministry of Education [M4360005]
- Institute for Collaborative Biotechnologies [W911NF-09-D-0001]
A novel conjugated oligoelectrolyte (COE) material, named S6, is designed to have a lipid-bilayer stabilizing topology afforded by an extended oligophenylenevinylene backbone. S6 intercalates biological membranes acting as a hydrophobic support for glycerophospholipid acyl chains. Indeed, Escherichia coli treated with S6 exhibits a twofold improvement in butanol tolerance, a relevant feature to achieve within the general context of modifying microorganisms used in biofuel production. Filamentous growth, a morphological stress response to butanol toxicity in E. coil, is observed in untreated cells after incubation with 0.9% butanol (v/v), but is mitigated by S6 treatment. Real-time fluorescence imaging using giant unilamellar vesicles reveals the extent to which S6 counters membrane instability. Moreover, S6 also reduces butanol-induced lipopolysaccharide release from the outer membrane to further maintain cell integrity. These findings highlight a deliberate effort in the molecular design of a chain-elongated COE to stabilize microbial membranes against environmental challenges.
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