期刊
ACS NANO
卷 13, 期 2, 页码 2004-2014出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b08475
关键词
photocatalysis; cellular response; RNA-seq; flagellar assembly; metabolic alteration
类别
资金
- National Natural Science Foundation of China-Henan Talents Fostering Joint Funds [U1504311]
- National Natural Science Foundation of China [21571160]
- Key Research Projects of the Science and Technology Department of Henan Province [182102210153]
Bacterial cells can be inactivated by external reactive oxygen species (ROS) produced by semiconductor photocatalysis. However, little is known about cellular responses to photocatalysis. For a better understanding of this issue, one strain of Escherichia colt (E. coli, hereafter named as MT), which has an increased ability to metabolize carbon sources, was screened out from the wild-type (WT) E. coli K12 by repeated exposure to photocatalysis with palladium oxide modified nitrogen-doped titanium dioxide. In this study, transcriptome sequencing of the WT and MT strains that were exposed or unexposed to photocatalysis were carried out. Cellular responses to photocatalysis were inferred from the functions of genes whose transcripts were either increased or decreased. Upregulation of expression of bacterial flagellar assembly genes used for chemotaxis was detected in cells exposed to semilethal photocatalytic conditions of the WT E. coli. Increased capability to degrade superoxide radicals and decreased bacterial flagellar assembly and chemotaxis were observed in MT E. coli compared to WT cells. We conclude that the differences in motility and intracellular ROS between MT and WT are directly related to survivability of E. coli during exposure to photodisinfection.
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