期刊
BIOFOULING
卷 31, 期 3, 页码 241-251出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2015.1028923
关键词
High-throughput sequencing (HTS); 18S rRNA gene; underwater sampling devices; Ciona savignyi; non-indigenous species (NIS); operational taxonomic unit (OTU)
资金
- National Institute of Water and Atmospheric Research under the Coasts and Oceans Research Programme 4, Marine Biosecurity [2013/14 SC]
Marine biofilms are precursors for colonization by larger fouling organisms, including non-indigenous species (NIS). In this study, high-throughput sequencing (HTS) of 18S rRNA metabarcodes was used to investigate four sampling methods (modified syringe, sterilized sponge, underwater tape and sterilized swab) for characterizing eukaryotic communities in marine biofilms. Perspex (TM) plates were sampled in and out of water. DNA collected with tape did not amplify. Otherwise, there were no statistical differences in communities among the remaining three sampling devices or between the two environments. Sterilized sponges are recommended for ease of use underwater. In-depth HTS analysis identified diverse eukaryotic communities, dominated by Metazoa and Chromoalveolata. Among the latter, diatoms (Bacillario-phyceae) were particularly abundant (33% of reads assigned to Chromalveolata). The NIS Ciona savignyi was detected in all samples. The application of HTS in marine biofilm surveillance could facilitate early detection of NIS, improving the probability of successful eradication.
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