Voluntary aerobic exercise increases arterial resilience and mitochondrial health with aging in mice

Mitochondrial dysregulation and associated excessive reactive oxygen species (mtROS) production is a key source of oxidative stress in aging arteries that reduces baseline function and may influence resilience (ability to withstand stress). We hypothesized that voluntary aerobic exercise would increase arterial resilience in old mice. An acute mitochondria! stressor (rotenone) caused greater (further) impairment in peak carotid EDD in old ((similar to)27 mo., OC, n=12; -32.5 +/--10.5%) versus young ((similar to)7 mo., YC n=11; -5.4 +/--3.7%) control male mice, whereas arteries from young and old exercising (YVR n=10 and OVR n=11, 10-wk voluntary running; -0.8 +/--2.1% and -8.0 +/- 4.9%, respectively) mice were protected. Ex-vivo simulated Western diet (WD, high glucose and palmitate) caused greater impairment in EDD in OC (-28.5 +/- 8.6%) versus YC (-16.9 +/- 5.2%) and YVR (-15.3 +/- 2.3%), whereas OVR (-8.9 +/- 3.9%) were more resilient (not different versus YC). Simultaneous ex-vivo treatment with mitochondria-specific antioxidant MitoQ attenuated WD-induced impairments in YC and OC, but not YVR or OVR, suggesting that exercise improved resilience to mtROS-mediated stress. Exercise normalized age-related alterations in aortic mitochondria! protein markers PGC-l alpha, SIRT-3 and Fis1 and augmented cellular antioxidant and stress response proteins. Our results indicate that arterial aging is accompanied by reduced resilience and mitochondria! health, which are restored by voluntary aerobic exercise.