期刊
BIOELECTROCHEMISTRY
卷 104, 期 -, 页码 10-16出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.12.003
关键词
Nitric oxide; Copper electrode; Antimicrobial catheters; Thromboresistant catheters; Modulated NO release
资金
- National Institutes of Health [NIH-EB-000783, NIH-R56-HL-119403-01]
Inexpensive nitric oxide (NO) release strategies to prevent thrombosis and bacterial infections are desirable for implantable medical devices. Herein, we demonstrate the utility of electrochemically modulated NO release from a catheter model using an inner copper wire working electrode and an inorganic nitrite salt solution reservoir. These catheters generate NO surface fluxes of >1.0 x 10(-10) mol min(-1) cm(-2) for more than 60 h. Catheters with an NO flux of 1.1 x 10(-1) mol min(-1) cm(-2) are shown to significantly reduce surface thrombus formation when implanted in rabbit veins for 7 h. Further, the ability of these catheters to exhibit anti-biofilm properties against bacterial species commonly causing bloodstream and urinary catheter infections is examined. Catheters releasing NO continuously during the 2 d growth of Staphylococcus aureus exhibit a 6 log-unit reduction in viable surface bacteria. We also demonstrate that catheters generating NO for only 3 hat a flux of 1.0 x 10(-10) mol cm(-2) lower the live bacterial counts of both 2 d and 4 d pre-formed Escherichia coli biofilms by >99.9%. Overall, the new electrochemical NO-release devices could provide a cost-effective strategy to greatly enhance the biocompatibility and antimicrobial properties of intravascular and urinary catheters, as well as other implantable medical devices. (C) 2014 Elsevier B.V. All rights reserved.
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