期刊
BIOELECTROCHEMISTRY
卷 101, 期 -, 页码 106-113出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.07.006
关键词
Human chitinase-3-like protein 1; Chitinase-like proteins; Capacitive immunosensor; Disease marker; Biomarker
资金
- Office of the Higher Education Commission, Thailand [CHE 510767]
- Suranaree University of Technology [SUT1-102-54-36-06]
- Prince of Songkla University funds for the Trace Analysis and Biosensor Research Center, Faculty of Science
Tissue inflammation, certain cardiovascular syndromes and the occurrence of some solid tumors are correlated with raised serum concentrations of human chitinase-3-like protein 1 (YKL-40), a mammalian chitinase-like glycoprotein, which has become the subject of current research. Here we report the construction and characterization of an electrochemical platform for label-free immunosensing of YKL-40. Details of the synthesis of YKL-40 and production of anti-YKL-40 immunoglobulin G (IgG) are provided and cross-reactivity tests presented. Polyclonal anti-YKL-40 IgG was immobilized on gold electrodes and the resulting immunosensors were operated in an electrochemical flow system with capacitive signal generation. The strategy offered a wide linear detection range (0.1 mu g/L to 1 mg/L) with correlation coefficients (R-2) above 0.99 and good sensitivity (12.28 +/- 0.27 nF/cm(2) per decade of concentration change). Additionally, the detection limit of 0.07 +/- 0.01 mu g/L was well below that of optical enzyme-linked immunosorbent assays (ELISAs), which makes the proposed methodology a promising alternative for YKL-40 related disease studies. (C) 2014 Elsevier B.V. All rights reserved.
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