期刊
BIOELECTROCHEMISTRY
卷 101, 期 -, 页码 92-96出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.08.012
关键词
Tethered bilayer lipid membrane; Electrochemical impedance spectroscopy; Dioleoylphosphatidylcholine; Gramicidin; Alamethicin
资金
- Italian Ministero dell'Istruzione, dell'Universita e della Ricerca (PRIN) [20098Z4M5E]
A biomimetic membrane consisting of a thiolipid monolayer tethered to a mercury electrode, with a dioleoylphosphatidylcholine (DOPC) monolayer on top of it, was fabricated. The thiolipid, referred to as DPOL, consisted of an octaethyleneoxy (OEO) chain terminated at one end with a lipoic acid residue and covalently linked at the other end to two phytanyl chains. The functionality of this biomimetic membrane, referred to as a tethered bilayer lipid membrane (tBLM), was tested by incorporating gramicidin and alamethicin and verifying their ion channel activity. Advantages and drawbacks with respect to a tBLM using a thiolipid, referred to as DPTL, with a tetraethyleneoxy (TEO) chain were examined by using electrochemical impedance spectroscopy, potential-step chronocoulometry and cyclic voltammetry. The maximum charge surface density of potassium ions stored in the OEO spacer amounts to 70 mu C cm(-2), as compared to a charge surface density of 45 mu C cm(-2) in the TEO spacer. The lipid bilayer moiety of the DPOL/DOPC tBLM is somewhat leakier than that of the DPTL/DOPC tBLM at potentials negative of about -0.65 V vs. the saturated calomel electrode. The estimated value of the surface dipole potential of the OEO spacer amounts to -0.180 V and is, therefore, smaller than that -0230 V, of the TEO spacer. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据